Region with audio plays in Designer, won't play on Display

I have a layout with a region that “autoplays” audio from a radio website. When I load the layout in Designer (which I am doing in Chrome on Windows), the audio begins playing immediately, as expected. When I load the layout on a Windows client, the audio will not play. I have setup the default browser on the client to Chrome, not sure what else is keeping the audio from starting/playing.

Thanks in advance for any help you can offer.

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