If you look at the top right of your screenshot next to “Required Files Status”, see that it says “Outside Download Window” 23:50-00:00
That means you’ve got it configured so that there’s only 10 minutes in the day where the player will be allowed to download content from the CMS. So as the Player doesn’t have the files it needs it’s unable to play the layout, or get the files it needs to play the layout.
Assuming you’re on 1.7.3, then you need to edit the Display Settings Profile for this display (or your default Windows profile) and set the download window to something more sensible.