Ok cool I am glad it’s working for you now 
Durations - after region with the longest duration will end then whole layout will reload, yes it should be about ~1s blink.
It’s fine ,you can have 24h duration, it may interfere with schedule, so if you’d have such problems you will know what could be causing them.
Twitter - well seems to be correctly configured, basically when I imported your layout to my CMS it was displaying tweets. (it would definitely could use some effect or text size/formatting but it was displaying tweets 
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So I wonder, perhaps curl might be a problem? (it should be (!) on Report fault page in CMS)
If it is then please see this topic Using Tickers, Forecast, Twitter and other external resources that make use of HTTPS connections
Do you perhaps use proxy? If yes then you might want to configure that in CMS (settings -> network tab) and on a player (Xibo Player Options -> advanced tab)